Chemical synthesis of oligonucleotides|Phosphoramidite Method|
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Chemical synthesis of oligonucleotides|Phosphoramidite Method|
Chemical synthesis of oligonucleotides|Phosphoramidite Method| Oligonucleotides are show segments of DNA/ RNA that have wide applications in mutagenesis, PCR, cloning and making screening libraries. Industrially a chemical synthesis called phosphoramidite method is employed. Detailed explanation of the same is given in the video! Enjoy & don't ...
58.1K viewsJun 10, 2021
Oligonucleotide Synthesis Methods
Sign up for Synthesis Summer today to save 50% off. For ages 8-14. | Synthesis
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Solid-Phase Peptide Synthesis: The Merrifield Method - SlideServe
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slideserve.com
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SOLVED:As shown in this section, oligonucleotide synthesis features as the key step a tetrazole-catalyzed coupling of an alcohol (the first nucleotide) with a phosphoramidite (the second nucleotide). On first sight (focusing on all the nitrogen lone pairs), the function of tetrazole appears to be that of a base (to deprotonate the alcohol), but this heterocycle is actually quite acidic (p Ka=4.8), about as much as acetic acid (Section 19-4). Moreover, kinetic experiments indicate a ratedetermini
SOLVED:As shown in this section, oligonucleotide synthesis features as the key step a tetrazole-catalyzed coupling of an alcohol (the first nucleotide) with a phosphoramidite (the second nucleotide). On first sight (focusing on all the nitrogen lone pairs), the function of tetrazole appears to be that of a base (to deprotonate the alcohol), but this heterocycle is actually quite acidic (p Ka=4.8), about as much as acetic acid (Section 19-4). Moreover, kinetic experiments indicate a ratedetermini
numerade.com
2 months ago
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