The novel Cleavage High-throughput Assay (CHiTA) developed at Penn State provides a scarless method to characterize thousands of diverse small self-cleaving RNA enzymes, called twister ribozymes, in a ...

Rapid progress in the sequencing of several genomes has provided abundant sequence information, but the functions of many of the genes remain to be determined. So, a simple and reliable knockdown tool ...

Small ribozymes such as Oryza sativa twister spontaneously cleave their own RNA when the ribozyme folds into its active conformation. The coupling between twister folding and self-cleavage has been ...

In new research published in the journal Angewandte Chemie, scientists applied a high throughput methodology to study the ribozyme in an innovative way. While work in this area has typically used ...

The “RNA world” hypothesis proposes that the earliest life on Earth may have been based on RNA — a single-stranded molecule similar in many ways to DNA — like some modern viruses. This is because, ...

Molecular model showing an RNA strand from a hepatitis D virus genomic ribozyme, complexed with a ribonucleoprotein. Science Photo Library / Alamy Stock Photo A genomic screen has revealed a new ...

The "RNA world" hypothesis proposes that the earliest life on Earth may have been based on RNA - a single-stranded molecule similar in many ways to DNA - like some modern viruses. This is because, ...

RNA molecules are an integral part of the human body: In cells, they ensure the transfer of genetic information and regulate the activity of genes. Some even act as catalysts, allowing chemical ...

Allosteric RNAs operate as molecular switches that alter folding and function in response to ligand binding. A common type of natural allosteric RNAs is the riboswitch; designer RNAs with similar ...

One serious limitation with current gene therapy strategies is a lack of control. When researchers deliver a gene to replace one linked to disease, there’s no way to turn on the therapeutic gene at ...

A new method can test the activity of thousands of RNA enzymes, called ribozymes, in a single experiment. The "RNA world" hypothesis proposes that the earliest life on Earth may have been based on RNA ...